ABSTRACT
The objective of this research is to describe and compare three different methods of generating ‘persona for lighting’ to envision users’ behaviour within the lighting environment. ‘Personas’ are used to represent typical users, highlighting their needs, perspectives, and expectations to aid user-centric design approaches. The researchers looked for the most useful method of shaping ‘personas for lighting’ to learn about users’ satisfaction with the various lighting conditions to identify their needs. Method one of lighting persona development, was based on interviews with 87 users of five buildings of four different types: an office, a primary school, two university buildings, and a factory. The lighting conditions were observed and measured in all the buildings. As a result, 22 personas for lighting were created. In method two personas were generated based on pre-interviews, workshops on lighting and post-interviews with ten users along with the onsite lighting measurements. Later, due to the Covid-19 pandemic’s lockdowns, an online survey on the visual lighting environment in home offices was carried out among 694 students and professionals from seven countries to create two more personas for lighting (method three). All 26 ‘personas for lighting’ were generated in relation to observed lighting conditions, based on the satisfaction, preferences and needs of the users working within variously lit indoor environments. All the tested methods can be used for nearly any type of building and room, but the resulting personas are different due to the specific limitations of the methods. The created personas may help to identify future users’ lighting preferences, needs and requirements and assist designers. However, to fully understand their impact on the lighting research practice they should be tested in real projects.
ABSTRACT
Preventing the progression to acute respiratory distress syndrome (ARDS) in COVID-19 is an unsolved challenge. The involvement of T cell immunity in this exacerbation remains unclear. To identify predictive markers of COVID-19 progress and outcome, we analyzed peripheral blood of 10 COVID-19-associated ARDS patients and 35 mild/moderate COVID-19 patients, not requiring intensive care. Using multi-parametric flow cytometry, we compared quantitative, phenotypic, and functional characteristics of circulating bulk immune cells, as well as SARS-CoV-2 S-protein-reactive T cells between the two groups. ARDS patients demonstrated significantly higher S-protein-reactive CD4+ and CD8+ T cells compared to non-ARDS patients. Of interest, comparison of circulating bulk T cells in ARDS patients to non-ARDS patients demonstrated decreased frequencies of CD4+ and CD8+ T cell subsets, with activated memory/effector T cells expressing tissue migration molecule CD11a++. Importantly, survival from ARDS (4/10) was accompanied by a recovery of the CD11a++ T cell subsets in peripheral blood. Conclusively, data on S-protein-reactive polyfunctional T cells indicate the ability of ARDS patients to generate antiviral protection. Furthermore, decreased frequencies of activated memory/effector T cells expressing tissue migratory molecule CD11a++ observed in circulation of ARDS patients might suggest their involvement in ARDS development and propose the CD11a-based immune signature as a possible prognostic marker.